Burst responses in field CA1 of hippocampal slices from SIRT1-KO and NeSTO mice. Two different protocols of TBS, followed by 40 min of test-pulse recording were used. A , 5 × 3 TBS, five bursts at 5 Hz (theta burst), each burst consisting of three pulses at 100 Hz. B , 10 × 10 TBS, 10 theta bursts, each burst consisting of 10 pulses at 100 Hz. The duration of each pulse within TBS was double that of the test pulse. For each burst response during LTP induction, the area under the curve was measured and each burst area was normalized to the first burst area. (The bottom panels of simulated responses were not scaled.) C, D SIRT1-KO (open circles) and WT (filled circles) burst responses elicited by 5 × 3 TBS ( C ) and 10 × 10 TBS ( D ). E, F , NeSTO (gray bars) and Nestin–Cre (black bars) burst responses elicited by 5 × 3 TBS ( E ) and 10 × 10 TBS ( F ) (p < 0.005, two-way ANOVA). Data represent mean ± SEM. 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