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► February (8) Experience ACNE STUDIOS Burst responses in field CA1 of hippocampal slices from SIRT1-KO and NeSTO mice. Two different protocols of TBS, followed by 40 min of test-pulse recording were used. A , 5 × 3 TBS, five bursts at 5 Hz (theta burst), each burst consisting of three pulses at 100 Hz. B , 10 × 10 TBS, 10 theta bursts, each burst consisting of 10 pulses at 100 Hz. The duration of each pulse within TBS was double that of the test pulse. For each burst response during LTP induction, the area under the curve was measured and each burst area was normalized to the first burst area. (The bottom panels of simulated responses were not scaled.) C, D SIRT1-KO (open circles) and WT (filled circles) burst responses elicited by 5 × 3 TBS ( C ) and 10 × 10 TBS ( D ). E, F , NeSTO (gray bars) and Nestin–Cre (black bars) burst responses elicited by 5 × 3 TBS ( E ) and 10 × 10 TBS ( F ) (p < 0.005, two-way ANOVA). Data represent mean ± SEM.
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SIRT1-KO mice show spatial learning and memory impairment. A , Success of SIRT1-KO mice (n = 5; open circles) to find the escape box in the Barnes maze was lower than for WT mice (n = 6; *p < 0.001; filled circles). B , Latency to find the escape box significantly decreased in WT mice compared with SIRT1-KO (*p < 0.001). C, D , Number of total errors ( C ) and deviations from the first error ( D ) (*p < 0.001) were both higher in SIRT1-KO mice than WT mice. E, F , Search strategy of WT ( E ) and SIRT1-KO ( F ) mice (serial strategy, yellow; spatial strategy, green; random strategy, red). Data represent means ± SEM. Statistical significance values correspond to the differences between curves analyzed all over the acquisition phase. *p < 0.05, days with statistical significance.
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