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A , Representative images of SIRT1-KO and WT Nissl-stained coronal brain sections. SIRT1-KO brains show normal gross neuroanatomy. B , Immunofluorescence of coronal brain sections from SIRT1-KO and WT mice. SIRT1 and NeuN are colocalized in WT hippocampal neurons in different areas, including the dentate gyrus, CA1, and CA3. SIRT1-KO sections show absence of SIRT1 in hippocampal neurons yet unaltered NeuN staining. Figure 7. 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