Highlights
Fast library prep and enrichment workflow
Provides a time-saving solution that is 85% faster than standard Illumina library prep and enrichment
Enhances library preparation efficiency with integrated protocols for blood and saliva
Enables advanced study designs in cancer research, genetic disease research, and whole-exome sequencing
Figures and Tables
Introduction
The Nextera Flex for Enrichment solution combines versatile, simple, and fast library prep and enrichment functionality for targeted enrichment and exome sequencing applications. It offers extraordinary flexibility for input type, input amount, and a wide range of supported enrichment sequencing applications encompassing custom panels, fixed panels, and whole-exome sequencing
Nextera Flex for Enrichment uses innovative bead-based chemistry with a simplified, single hybridization step
Fast and flexible library preparation and enrichment workflow
A key component of the Nextera Flex for Enrichment solution is On-Bead Tagmentation, which uses bead-bound transposomes to mediate a uniform tagmentation reaction. This strategy provides several significant advantages.
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For gDNA inputs ≥ 50 ng, accurate quantitation of the initial DNA sample is not required, as insert fragment size is not affected, saving time and costs associated with kits and reagents.
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On-Bead Tagmentation eliminates the need for separate DNA fragmentation steps, saving time and costs associated with related consumables.
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For gDNA inputs between 50–1000 ng, saturation-based DNA normalization eliminates the need for individual library quantitation and normalization steps before enrichment.
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Novel 90-minute single hybridization protocol enables enrichment in less than four hours.
Fastest Illumina enrichment workflow
The Nextera Flex for Enrichment solution supports liquid-handling systems for library prep automation and produces a workflow with the lowest number of steps and the fastest total workflow time in the Illumina portfolio
Integrated DNA Input
DNA extraction can be processed directly from whole blood or saliva samples. The optional Flex Lysis Reagent Kit has been optimized and validated for Nextera Flex for Enrichment library preparation from whole blood and is integrated into the workflow for maximum efficiency. The lysis protocols feature bead-based reagents and require less than 30 minutes of hands-on time.
Optimized performance across Illumina sequencing systems
The robust and straightforward Nextera Flex for Enrichment solution yields reliable results across all Illumina sequencing systems by providing > 90% on-target reads, > 95% uniformity, and low PCR duplicate rate
DNA enrichment for a broad range of applications
By combining exceptional enrichment performance and the proven accuracy of Illumina sequencing by synthesis (SBS) chemistry,1 the Nextera Flex for Enrichment solution supports both fixed and custom panels of varying sizes, including whole-exome sequencing, for customers who need advanced study designs in a variety of areas
Accurate Data
Nextera Flex for Enrichment produces highly uniform and consistent insert sizes, across a wide DNA input range, delivering uniform and consistent library yields.2 In addition, Nextera Flex for Enrichment provides high coverage uniformity and padded read enrichment for custom, fixed, and exome panels
Summary
Nextera Flex for Enrichment features the fastest workflow in the Illumina enrichment portfolio. The user-friendly, automation-compatible solution supports users of all experience levels and provides a common workflow for a variety of experimental designs. On-Bead Tagmentation chemistry enables support for a wide range of DNA input amounts, various sample types, and a broad range of applications, including fixed panels, custom panels, and whole-exome sequencing. Furthermore, Nextera Flex for Enrichment is compatible with Illumina and third-party enrichment probes/panels, which enables content portability. The innovative Nextera Flex for Enrichment solution combined with the power of Illumina SBS chemistry provides an optimal targeted enrichment and exome sequencing experience.
Ordering Information
Product |
Catalog No. |
---|---|
Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents |
20025523 |
Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents |
20025524 |
Nextera DNA Flex Pre-Enrichment Library Prep Reagents (16 samples) |
20025519 |
Nextera DNA Flex Pre-Enrichment Library Prep Reagents (96 samples) |
20025520 |
Flex Lysis Reagent Kit (for blood lysis) |
20018706 |
IDT for Illumina - Nextera DNA Unique Dual Indexes - Set A (96 indexes, 96 samples) |
20027213 |
Illumina Exome Panel (8 enrichment reactions) |
20020183 |
TruSight One (6 enrichment reactions) |
20029227 |
TruSight One Expanded (6 enrichment reactions) |
20029226 |
TruSight Cancer (8 enrichment reactions) |
FC-121-0202 |
TruSight Hereditary Cancer (8 enrichment reactions) |
20029551 |
TruSight Cardio (1 enrichment reaction) |
20029228 |
TruSight Cardio (8 enrichment reactions) |
20029229 |
TruSeq Neurodegeneration (8 enrichment reactions) |
20029550 |
Illumina Custom Enrichment Panel |
20025371 |
References
1. | Bentley DR, Balasubramanian S, Swerdlow HP, et al. Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 2008;456:53-59. |
2. | Illumina (2017). Nextera DNA Flex Library Preparation Kit Data Sheet. Accessed September 10, 2018. |
Additional Resources
Library Prep and Array Kit Selector
This tool will help you determine the best kit for your needs based on your project type, starting material, and the sequencing method or application.
Custom Protocol Selector
Custom Protocol Selector is now faster, mobile-friendly, and even more flexible. Customize your level of detail, add notes, and print directly from the browser.
Illumina Concierge Services
Access a range of support options including advanced content design, assistance assessing custom enrichment, in-person training, and more.
Illumina, Inc. • 1.800.809.4566 toll-free (US) • +1.858.202.4566 tel • techsupport@illumina.com • www.illumina.com © 2018 Illumina, Inc. All rights reserved. All trademarks are the property of Illumina, Inc. or their respective owners. For specific trademark information, see www.illumina.com/company/legal.html. |
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