LOCUS AY562539 7287 bp DNA circular SYN 05-APR-2004 DEFINITION Binary Vector pORE_O1, complete sequence. ACCESSION AY562539 VERSION AY562539.1 GI:45862361 KEYWORDS . SOURCE Binary vector pORE_O1 ORGANISM Binary vector pORE_O1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7287) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE pORE: a new series of binary vectors with a removable selectable marker and 21 site MCS JOURNAL Unpublished REFERENCE 2 (bases 1 to 7287) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE Direct Submission JOURNAL Submitted (01-MAR-2004) Molecular Genetics, Agriculture and Agrifood Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada FEATURES Location/Qualifiers source 1..7287 /organism="Binary vector pORE_O1" /mol_type="other DNA" /db_xref="taxon:267416" /lab_host="Agrobacterium tumefaciens" terminator 1..253 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" misc_recomb 260..307 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" misc_feature 356..3610 /note="derived from pCB301 in GenBank Accession Number AF139061; used SDM to remove NotI, XhoI, EcoRV and SfiI sites" misc_feature 356..516 /note="T-DNA left border" rep_origin 523..1152 /note="RK2 oriV" CDS 1364..2158 /note="neomycin-kanamycin phosphotransferase type III" /codon_start=1 /transl_table=11 /product="NptIII" /protein_id="AAS78948.1" /db_xref="GI:45862362" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" CDS 2457..3605 /note="derived from plasmid RK2" /codon_start=1 /transl_table=11 /product="TrfA" /protein_id="AAS78949.1" /db_xref="GI:45862363" /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG SMVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKK RKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALF TTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPI TFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLD RGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKL ETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" rep_origin 3663..4543 /note="ColE1; high copy number origin of replication" misc_feature 4549..4711 /note="T-DNA right border" primer_bind 4724..4746 /note="M13/pUC forward" /PCR_conditions="60 degree C annealing temperature" misc_feature 4747..4890 /note="multiple cloning site; selected unique sites: SfoI-SacII-HindIII-NheI-MfeI-PspOMI-AclI-XhoI-XbaI-StuI- BamHI-SmaI-EcoRI-SalI-NotI-ClaI-PstI-SacI-KpnI-SpeI-EcoRV" primer_bind complement(4891..4913) /note="M13/pUC reverse" /PCR_conditions="60 degree C annealing temperature" terminator 4920..5172 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" misc_recomb 5179..5226 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" promoter 5243..6719 /note="At4g15440 promoter; hydroperoxide lyase promoter; constitutive; high level expression of transgenes in dicots; active in Arabidopsis, tobacco, cauliflower, soybean, alfalfa, peach, white spruce, wheat, and grape; in Arabidopsis, expression has been detected in all tissues but the petals, style, and pollen; removed ClaI, SfoI, XhoI and MfeI sites" CDS 6728..7279 /note="phosphinothricin acetyltransferase II; removed AatII, AvrII, BamHI, EcoRV, PspOMI, SacII, SmaI, StuI and XbaI sites" /codon_start=1 /transl_table=11 /product="PAT" /protein_id="AAS78950.1" /db_xref="GI:45862364" /translation="MSPERRPVEIRPATAADMAAVCDIVNHYIETSTVNFRTEPQTPQ EWIDDLERLQDRYPWLVAEVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGL GSTLYTHLLKSMEAQGFKSVVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHD VGFWQRDFELPAPPRPVRPVTQI" ORIGIN 1 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 61 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 121 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 181 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 241 atgttactag atccctaggg aagttcctat tccgaagttc ctattctctg aaaagtatag 301 gaacttcttt gcgtattggg cgctcttggc ctttttggcc accggtcgta cggttaaaac 361 caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt 421 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca 481 caaaatcacc actcgataca ggcagcccat cagtccacta gacgctcacc gggctggttg 541 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga 601 agccgtgtgc gagacaccgc agccgccggc gttgtggata cctcgcggaa aacttggccc 661 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt 721 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa 781 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga 841 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat 901 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg 961 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc 1021 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg 1081 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc 1141 tcccggcccg ctctcgcgtt ggcagcatca cccataattg tggtttcaaa atcggctccg 1201 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt 1261 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt 1321 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat 1381 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat 1441 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac 1501 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg 1561 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag 1621 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca 1681 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga 1741 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt 1801 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa 1861 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt 1921 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt 1981 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg 2041 gtcgatcagg gaggatattg gggaagaaca gtatgtcgag ctattttttg acttactggg 2101 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta 2161 cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca 2221 agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat 2281 tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga 2341 ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc 2401 aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga 2461 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg 2521 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt 2581 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg 2641 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg 2701 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga 2761 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caagccgcgt 2821 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc 2881 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca 2941 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca 3001 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt 3061 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt 3121 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg 3181 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc 3241 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt 3301 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca 3361 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact 3421 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg 3481 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt 3541 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac 3601 gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct ttactgagat 3661 cctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttggatctc ctgtggttgg 4561 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta 4621 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat 4681 agtttaaact gaaggcggga aacgacaatc tgctagtgga tctcccagtc acgacgttgt 4741 aaaacgggcg ccccgcggaa agcttgctag ccaattgggg cccaacgttc tcgagttttt 4801 ctagaaggcc ttggatccac ccgggagaat tcgtcgactt tgcggccgca tcgatactgc 4861 aggagctcgg taccttttac tagtgatatc cctgtgtgaa attgttatcc gctacgcgtg 4921 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga 4981 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca 5041 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg 5101 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta 5161 tgttactaga tcccatggga agttcctatt ccgaagttcc tattctctga aaagtatagg 5221 aacttcagcg atcgcagacg tcaacgtgga tacttggcag tggttacttg gcttttcctt 5281 tattttcttt tggacggaag cggtggttac tttgtcacac atttaaaaaa acacgtgttt 5341 ctcacttttt tctattcccg tcacaaacaa ttttaagaaa gatccatcta tcgtgatctt 5401 tctatcaaac aaaagaaaaa aggtcttcat agtaacgcta caacatcaaa tatgtggttg 5461 ctctgacatc agtcgggaaa ataaggatat ggcggcattg gccacatcta ttggggtccc 5521 aacttccttt cacaaaaaaa ttaaattggg tgtcccaact tttatctttg atatagtgac 5581 atgagtatcg ggagcattgg acaatggata aaatgagaac taaaaaaatt ctggttaatt 5641 tttgatcatt gttatttaaa aggttatttt atctataatc tacccatatt gatcagtttt 5701 atttaaattt gtttagctac cgctccacga gagagatcct catcttaaaa atggaatatg 5761 gaaattacac acgaccccaa aagtatattt tttctctgga gaatgctatt tagagctttg 5821 actatatggt ctgaattaga aagacgggaa ataaaatctg ctaagtgata taagctctaa 5881 gtaggcgatg tgtgatggag aacacctttt ctttaacagt cttcatgttt tacagattcg 5941 cgaacttcga atatccctat acggtctgtc taaccctcgt gtgtcttttg agtccaagat 6001 aaaggccatt attgagtaac atagacatgc tggaatccaa ccattgaagt cacaactgtc 6061 catgtagatt ctttggagaa tctgaaaagt cttaataaag gtggtgtttc aaagaaaaca 6121 aaacaaatga gttaagaaaa aaaaatatca tgtagtggtc gagtattatg ttatttattg 6181 tgtagctacc aatctttatt ctttaaatct gacataaaat gctacaaact ttttacctcg 6241 tctatagccc caaaaaacct aaccacggtt ctaaaaccac acacagtgat tttggttgac 6301 gacaatgcct ctccttcctc aaaacgattt atttacattt tttaaatcaa atgttacatt 6361 ttataccata attaagtctt tttacagaat acttagatgg aagagatgta taaaaaagga 6421 ggaaattgta aaaaacatat ttcgatcaat taaaccagga ttcataaaaa tataagtata 6481 tatataaatg atgtttcgtt tagcgatgaa cttcactcat atgataatac ttaacaatat 6541 aagtacataa aaaataaaat aaaattaatt gtttacgaaa agtctacaaa tactgcatgt 6601 ataattaatg ttctctttat ttatttattt ataccttacc aagatatatc tataaccgca 6661 tagaaataga aggcgaagag ataatttcca aaaacaagaa aaacctctaa gctcaaaagg 6721 gccggccatg tctccggaga ggagaccagt tgagattagg ccagctacag cagctgatat 6781 ggccgctgtt tgtgacatcg ttaaccatta cattgagact tctacagtga actttaggac 6841 agagccacaa acaccacaag agtggattga tgatcttgag aggttgcaag atagataccc 6901 ttggttggtt gctgaggttg agggtgttgt ggctggtatt gcttacgctg gaccttggaa 6961 ggctaggaac gcttacgatt ggacagttga gagtactgtt tacgtgtcac ataggcatca 7021 aaggttgggc ctcggatcta cattgtacac acatttgctt aagtctatgg aggcgcaagg 7081 ttttaagtct gtggttgctg ttattggcct tccaaacgat ccatctgtta ggttgcatga 7141 ggctttggga tacacagcca ggggtacatt gcgcgcagct ggatacaagc atggtggatg 7201 gcatgatgtt ggtttttggc aaagggattt tgagttgcca gctcctccaa ggccagttag 7261 accagttacc cagatctgag gcgcgcc //