LOCUS sequences_3_ 7208 bp ds-DNA circular 21-JUN-2010 DEFINITION Binary Vector pORE_O1, complete sequence. ACCESSION AY562539 VERSION AY562539.1 GI:45862361 KEYWORDS . SOURCE Binary vector pORE_O1 ORGANISM Binary vector pORE_O1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7287) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE pORE: a new series of binary vectors with a removable selectable marker and 21 site MCS JOURNAL Unpublished REFERENCE 2 (bases 1 to 7287) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE Direct Submission JOURNAL Submitted (01-MAR-2004) Molecular Genetics, Agriculture and Agrifood Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source join(1..4756,4801..7208) /organism="Binary vector pORE_O1" /mol_type="other DNA" /db_xref="taxon:267416" /lab_host="Agrobacterium tumefaciens" /label=source:Binary vector pORE_O1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink terminator 1..253 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" /label=NOS terminator; polyadenylation signal from nopali /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_recomb 260..307 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" /label=FRT site; excision of selectable marker cassette f /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 356..3610 /note="derived from pCB301 in GenBank Accession Number AF139061; used SDM to remove NotI, XhoI, EcoRV and SfiI sites" /label=derived from pCB301 in GenBank Accession Number AF /ApEinfo_fwdcolor=#edff00 /ApEinfo_revcolor=green misc_feature 356..516 /note="T-DNA left border" /label=T-DNA left border /ApEinfo_fwdcolor=#edff00 /ApEinfo_revcolor=green rep_origin 523..1152 /note="RK2 oriV" /label=RK2 oriV /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 1364..2158 /note="neomycin-kanamycin phosphotransferase type III" /codon_start=1 /transl_table=11 /product="NptIII" /protein_id="AAS78948.1" /db_xref="GI:45862362" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" /label=NptIII /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 2457..3605 /note="derived from plasmid RK2" /codon_start=1 /transl_table=11 /product="TrfA" /protein_id="AAS78949.1" /db_xref="GI:45862363" /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG SMVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKK RKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALF TTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPI TFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLD RGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKL ETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" /label=TrfA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin 3663..4543 /note="ColE1; high copy number origin of replication" /label=ColE1; high copy number origin of replication /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4549..4711 /note="T-DNA right border" /label=T-DNA right border /ApEinfo_fwdcolor=#edff00 /ApEinfo_revcolor=green primer_bind 4724..4746 /note="M13/pUC forward" /PCR_conditions="60 degree C annealing temperature" /label=M13/pUC forward /ApEinfo_fwdcolor=#efff00 /ApEinfo_revcolor=green misc_feature join(4747..4756,4801..4811) /note="multiple cloning site; selected unique sites: SfoI-SacII-HindIII-NheI-MfeI-PspOMI-AclI-XhoI-XbaI-StuI- BamHI-SmaI-EcoRI-SalI-NotI-ClaI-PstI-SacI-KpnI-SpeI-EcoRV" /label=multiple cloning site; selected unique sites: SfoI /ApEinfo_fwdcolor=#edff00 /ApEinfo_revcolor=green primer_bind complement(4812..4834) /note="M13/pUC reverse" /PCR_conditions="60 degree C annealing temperature" /label=M13/pUC reverse /ApEinfo_fwdcolor=#efff00 /ApEinfo_revcolor=green terminator 4841..5093 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" /label=NOS terminator; polyadenylation signal from nopali(1) /ApEinfo_label=NOS terminator; polyadenylation signal from nopali /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_recomb 5100..5147 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" /label=FRT site; excision of selectable marker cassette f(1) /ApEinfo_label=FRT site; excision of selectable marker cassette f /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter 5164..6640 /note="At4g15440 promoter; hydroperoxide lyase promoter; constitutive; high level expression of transgenes in dicots; active in Arabidopsis, tobacco, cauliflower, soybean, alfalfa, peach, white spruce, wheat, and grape; in Arabidopsis, expression has been detected in all tissues but the petals, style, and pollen; removed ClaI, SfoI, XhoI and MfeI sites" /label=At4g15440 promoter; hydroperoxide lyase promoter; /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 6649..7200 /note="phosphinothricin acetyltransferase II; removed AatII, AvrII, BamHI, EcoRV, PspOMI, SacII, SmaI, StuI and XbaI sites" /codon_start=1 /transl_table=11 /product="PAT" /protein_id="AAS78950.1" /db_xref="GI:45862364" /translation="MSPERRPVEIRPATAADMAAVCDIVNHYIETSTVNFRTEPQTPQ EWIDDLERLQDRYPWLVAEVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGL GSTLYTHLLKSMEAQGFKSVVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHD VGFWQRDFELPAPPRPVRPVTQI" /label=PAT /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 4759..4799 /label=biobrick multiple cloning site /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 61 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 121 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 181 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 241 atgttactag atccctaggg aagttcctat tccgaagttc ctattctctg aaaagtatag 301 gaacttcttt gcgtattggg cgctcttggc ctttttggcc accggtcgta cggttaaaac 361 caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt 421 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca 481 caaaatcacc actcgataca ggcagcccat cagtccacta gacgctcacc gggctggttg 541 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga 601 agccgtgtgc gagacaccgc agccgccggc gttgtggata cctcgcggaa aacttggccc 661 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt 721 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa 781 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga 841 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat 901 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg 961 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc 1021 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg 1081 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc 1141 tcccggcccg ctctcgcgtt ggcagcatca cccataattg tggtttcaaa atcggctccg 1201 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt 1261 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt 1321 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat 1381 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat 1441 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac 1501 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg 1561 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag 1621 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca 1681 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga 1741 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt 1801 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa 1861 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt 1921 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt 1981 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg 2041 gtcgatcagg gaggatattg gggaagaaca gtatgtcgag ctattttttg acttactggg 2101 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta 2161 cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca 2221 agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat 2281 tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga 2341 ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc 2401 aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga 2461 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg 2521 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt 2581 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg 2641 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg 2701 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga 2761 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caagccgcgt 2821 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc 2881 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca 2941 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca 3001 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt 3061 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt 3121 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg 3181 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc 3241 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt 3301 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca 3361 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact 3421 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg 3481 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt 3541 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac 3601 gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct ttactgagat 3661 cctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttggatctc ctgtggttgg 4561 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta 4621 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat 4681 agtttaaact gaaggcggga aacgacaatc tgctagtgga tctcccagtc acgacgttgt 4741 aaaacgggcg ccccgcGGGA ATTCGCGGCC GCTTCTAGAA CTAGTAGCGG CCGCTGCAGG 4801 ctagtgatat ccctgtgtga aattgttatc cgctacgcgt gatcgttcaa acatttggca 4861 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct 4921 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg 4981 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata 5041 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcccatggg 5101 aagttcctat tccgaagttc ctattctctg aaaagtatag gaacttcagc gatcgcagac 5161 gtcaacgtgg atacttggca gtggttactt ggcttttcct ttattttctt ttggacggaa 5221 gcggtggtta ctttgtcaca catttaaaaa aacacgtgtt tctcactttt ttctattccc 5281 gtcacaaaca attttaagaa agatccatct atcgtgatct ttctatcaaa caaaagaaaa 5341 aaggtcttca tagtaacgct acaacatcaa atatgtggtt gctctgacat cagtcgggaa 5401 aataaggata tggcggcatt ggccacatct attggggtcc caacttcctt tcacaaaaaa 5461 attaaattgg gtgtcccaac ttttatcttt gatatagtga catgagtatc gggagcattg 5521 gacaatggat aaaatgagaa ctaaaaaaat tctggttaat ttttgatcat tgttatttaa 5581 aaggttattt tatctataat ctacccatat tgatcagttt tatttaaatt tgtttagcta 5641 ccgctccacg agagagatcc tcatcttaaa aatggaatat ggaaattaca cacgacccca 5701 aaagtatatt ttttctctgg agaatgctat ttagagcttt gactatatgg tctgaattag 5761 aaagacggga aataaaatct gctaagtgat ataagctcta agtaggcgat gtgtgatgga 5821 gaacaccttt tctttaacag tcttcatgtt ttacagattc gcgaacttcg aatatcccta 5881 tacggtctgt ctaaccctcg tgtgtctttt gagtccaaga taaaggccat tattgagtaa 5941 catagacatg ctggaatcca accattgaag tcacaactgt ccatgtagat tctttggaga 6001 atctgaaaag tcttaataaa ggtggtgttt caaagaaaac aaaacaaatg agttaagaaa 6061 aaaaaatatc atgtagtggt cgagtattat gttatttatt gtgtagctac caatctttat 6121 tctttaaatc tgacataaaa tgctacaaac tttttacctc gtctatagcc ccaaaaaacc 6181 taaccacggt tctaaaacca cacacagtga ttttggttga cgacaatgcc tctccttcct 6241 caaaacgatt tatttacatt ttttaaatca aatgttacat tttataccat aattaagtct 6301 ttttacagaa tacttagatg gaagagatgt ataaaaaagg aggaaattgt aaaaaacata 6361 tttcgatcaa ttaaaccagg attcataaaa atataagtat atatataaat gatgtttcgt 6421 ttagcgatga acttcactca tatgataata cttaacaata taagtacata aaaaataaaa 6481 taaaattaat tgtttacgaa aagtctacaa atactgcatg tataattaat gttctcttta 6541 tttatttatt tataccttac caagatatat ctataaccgc atagaaatag aaggcgaaga 6601 gataatttcc aaaaacaaga aaaacctcta agctcaaaag ggccggccat gtctccggag 6661 aggagaccag ttgagattag gccagctaca gcagctgata tggccgctgt ttgtgacatc 6721 gttaaccatt acattgagac ttctacagtg aactttagga cagagccaca aacaccacaa 6781 gagtggattg atgatcttga gaggttgcaa gatagatacc cttggttggt tgctgaggtt 6841 gagggtgttg tggctggtat tgcttacgct ggaccttgga aggctaggaa cgcttacgat 6901 tggacagttg agagtactgt ttacgtgtca cataggcatc aaaggttggg cctcggatct 6961 acattgtaca cacatttgct taagtctatg gaggcgcaag gttttaagtc tgtggttgct 7021 gttattggcc ttccaaacga tccatctgtt aggttgcatg aggctttggg atacacagcc 7081 aggggtacat tgcgcgcagc tggatacaag catggtggat ggcatgatgt tggtttttgg 7141 caaagggatt ttgagttgcc agctcctcca aggccagtta gaccagttac ccagatctga 7201 ggcgcgcc //