LOCUS New_DNA 8277 bp ds-DNA linear 03-JAN-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT /Users/nkuldell/Desktop/pSB1A2.ape from 1 to 2079 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin complement(159..841) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 misc_feature 2037..2056 /label=BB prefix (RI, Xba) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green CDS complement(939..1598) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow misc_feature 8257..8277 /label=BB suffix (Spe, Pst) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green ORIGIN 1 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 61 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 1981 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat 2041 tcgcggccgc ttctagttaa ggaggtaaaa aaaatgaaac attcttccga tatctgcatt 2101 gttggtgctg gtatttctgg tttgacgtgc gcaagccatc tgctggacag cccggcatgc 2161 cgtggtctga gcctgcgtat ctttgacatg cagcaagaag ccggtggccg tatccgcagc 2221 aaaatgctgg atggtaaggc aagcattgaa ctgggcgcag gtcgctactc ccctcagttg 2281 cacccgcatt tccaaagcgc aatgcagcac tatagccaaa agagcgaagt ctatccgttc 2341 acccagttga agttcaaatc tcacgtgcag caaaagctga agcgcgccat gaatgaactg 2401 tccccgcgtc tgaaagagca tggtaaagag agctttttgc agtttgtcag ccgttatcaa 2461 ggtcacgata gcgcggttgg tatgatccgc tctatgggtt acgacgcact gttcctgccg 2521 gatatcagcg cagaaatggc ctacgacatt gtgggtaagc acccggagat ccagagcgtg 2581 acggacaacg acgcgaacca atggtttgca gcggaaacgg gctttgctgg tctgattcag 2641 ggcatcaagg ctaaggttaa ggcggcaggt gcgcgtttta gcctgggtta tcgtctgctg 2701 agcgtccgta ccgacggtga cggctacctg ctgcaactgg caggtgacga cggctggaaa 2761 ctggagcacc gtacccgcca tctgattctg gcgattccgc cgagcgcgat ggcgggtttg 2821 aatgttgatt ttccagaagc ctggtccggt gcgcgctatg gcagcctgcc gctgtttaag 2881 ggctttctga cgtacggtga gccgtggtgg ttggactaca aactggacga tcaggtgctg 2941 attgttgaca acccgctgcg caaaatctat ttcaaaggcg ataagtacct gttcttctat 3001 accgatagcg agatggcgaa ttactggcgc ggttgtgtcg cggagggcga ggacggttac 3061 ctggagcaaa ttcgcaccca tttggctagc gcactgggta tcgtccgtga acgtatcccg 3121 caaccgctgg cacacgttca caagtattgg gcgcacggcg ttgagttttg ccgtgattct 3181 gatattgacc acccgagcgc actgtctcat cgcgacagcg gtatcatcgc gtgctccgat 3241 gcgtacacgg agcattgtgg ttggatggag ggcggtctgc tgagcgcccg tgaggcaagc 3301 cgtctgctgt tgcagcgtat cgccgcgtga ttaaggaggt aaaaaaaatg agcattctgg 3361 atttcccgcg tatccacttc cgtggctggg cccgtgtcaa tgcgccgacc gcgaaccgcg 3421 atccgcacgg ccacatcgat atggccagca ataccgtggc gatggcgggt gagccgttcg 3481 acctggcacg ccatcctacg gagttccacc gtcacctgcg ctccctgggt ccgcgcttcg 3541 gcttggatgg tcgtgctgac ccggaaggcc cgttcagcct ggccgagggc tacaacgctg 3601 ccggtaacaa ccacttttcg tgggagagcg caaccgttag ccacgtgcaa tgggatggcg 3661 gtgaggcgga tcgtggtgac ggtctggtcg gtgctcgttt ggcactgtgg ggtcactaca 3721 atgattatct gcgtaccacc ttcaatcgtg ctcgttgggt cgacagcgac ccgacgcgcc 3781 gtgacgctgc acaaatctat gcgggccaat tcaccattag cccggctggt gccggtccgg 3841 gtacgccgtg gctgtttacg gcagacattg atgatagcca tggtgcacgt tggacgcgtg 3901 gcggccacat tgcagagcgt ggcggccact tcttggatga agagtttggt ctggcacgcc 3961 tgtttcagtt ctctgtgccg aaagatcacc cacattttct gtttcacccg ggtccgtttg 4021 attccgaggc ctggcgtcgt ctgcaattgg ctctggagga tgacgacgtt ctgggtctga 4081 ccgtgcaata tgcgttgttc aatatgagca ccccgcctca gccgaacagc ccggtttttc 4141 acgatatggt cggtgttgtc ggtctgtggc gtcgtggtga actggcgagc tacccggctg 4201 gtcgtctgct gcgtccgcgt caaccgggtc tgggtgacct gaccctgcgc gtcaacggtg 4261 gtcgcgttgc gctgaatttg gcgtgtgcca ttccgttcag cactcgtgcc gcgcagccaa 4321 gcgcaccgga ccgcctgacc ccggacctgg gtgccaaact gccgctgggc gatctgctgc 4381 tgcgtgatga ggacggcgca ctgttggcac gtgtgccgca ggctctgtac caagactatt 4441 ggacgaatca cggtattgtg gacctgccgc tgctgcgcga accgcgtggt agcttgaccc 4501 tgagcagcga actggcggag tggcgtgagc aagactgggt cacccaaagc gacgcgtcta 4561 acctgtacct ggaggcaccg gatcgccgtc acggtcgctt tttccctgag agcatcgcgc 4621 tgcgcagcta ctttcgcggt gaagcgcgtg cgcgtccgga tatcccgcat cgtatcgagg 4681 gcatgggcct ggtcggcgtc gaatctcgtc aggatggcga cgctgcggaa tggcgtctga 4741 cgggtctgcg tccgggtccg gcacgcattg ttctggacga tggtgccgag gcgatccctc 4801 tgcgtgttct gcctgacgat tgggcgctgg atgacgcgac cgtcgaagaa gtggattacg 4861 cctttttgta ccgccacgtt atggcgtatt acgagctggt gtatccattc atgagcgaca 4921 aggtgttttc cctggctgat cgttgcaaat gtgaaacgta cgcacgtctg atgtggcaga 4981 tgtgtgatcc gcagaaccgc aacaagtcct attacatgcc gagcacccgc gaactgtcgg 5041 caccgaaagc tcgtttgttc ttgaagtatc tggcccacgt ggaaggccag gcacgcctgc 5101 aagcacctcc gccagcgggt ccggcacgca ttgaatctaa agcccagttg gcggcagagc 5161 tgcgtaaagc cgtcgacctg gagctgtctg tgatgctgca atacctgtac gcggcgtata 5221 gcattccgaa ctatgcacag ggccaacaac gtgttcgtga cggtgcgtgg accgccgagc 5281 agctgcaact ggcgtgcggt agcggtgacc gtcgccgtga tggcggtatt cgtgcagcac 5341 tgctggaaat tgctcatgaa gaaatgattc attacctggt cgttaacaac ctgctgatgg 5401 ccctgggcga gccgttctac gcgggtgtcc cgctgatggg cgaagcggca cgtcaggcgt 5461 ttggcctgga caccgagttc gctctggaac cgtttagcga aagcacgctg gcacgttttg 5521 ttcgtctgga atggccgcac tttatcccag caccgggcaa atccatcgcg gactgctatg 5581 ccgccattcg tcaggcgttt ttggatctgc cggacttgtt tggtggcgag gcaggtaagc 5641 gtggcggtga acaccacctg ttcctgaatg agctgaccaa ccgtgcgcat ccgggttatc 5701 aactggaagt tttcgatcgc gactcggcgc tgtttggtat tgcatttgtg accgatcagg 5761 gcgaaggtgg cgctctggac agcccgcact acgaacatag ccattttcaa cgtctgcgtg 5821 aaatgagcgc gcgtatcatg gctcaaagcg caccgttcga accggcgctg ccggcgttgc 5881 gtaatccggt tctggatgag agcccgggtt gccaacgtgt cgcagacggt cgtgcgcgtg 5941 cgctgatggc attgtaccaa ggcgtttatg agctgatgtt tgcgatgatg gcgcagcact 6001 tcgccgtgaa accgctgggt agcttgcgtc gcagccgcct gatgaacgca gcaatcgatc 6061 tgatgaccgg tctgttgcgt ccgctgagct gcgcgctgat gaacctgcca agcggcatcg 6121 ccggtcgcac ggccggtccg ccgctgccgg gtccggttga cacccgtagc tatgacgact 6181 acgcgctggg ctgtcgcatg ctggcacgcc gttgcgagcg tctgctggag caggcgagca 6241 tgctggaacc gggttggctg ccggatgcgc agatggagct gctggatttc tatcgtcgcc 6301 aaatgctgga cttggcgtgc ggcaaactga gccgcgaggc ctaaggatcc ttaaggaggt 6361 aaaaaaaatg aaacgtgcga ttatcgttgg tggcggcctg gcgggtggcc tgaccgcgat 6421 ctacctggcg aagcgtggct acgaagtgca cgtcgtggag aagcgtggtg atcctctgcg 6481 cgatctgagc tcttacgtgg acgttgttag cagccgtgcg atcggcgtga gcatgaccgt 6541 tcgtggtatc aagagcgttt tggctgcggg cattccgcgt gcagagctgg atgcgtgtgg 6601 cgaaccgatc gtggcaatgg ctttctccgt gggtggtcag tatcgcatgc gcgaactgaa 6661 gccgttggag gatttccgtc cgctgagctt gaaccgtgcg gcgtttcaaa agctgctgaa 6721 caaatacgcg aacctggcag gcgttcgtta ctactttgag cataagtgcc tggatgttga 6781 cctggatggt aagagcgtgt tgattcaggg caaagatggt cagccgcagc gtctgcaagg 6841 tgacatgatt atcggtgcgg atggcgccca cagcgccgtc cgtcaggcga tgcagagcgg 6901 cctgcgtcgt ttcgagttcc agcaaacgtt cttccgccat ggctacaaaa ccctggtttt 6961 gccggacgcg caagcactgg gttaccgtaa agacacgctg tactttttcg gcatggattc 7021 cggtggcctg ttcgcgggtc gtgcggctac gatcccagat ggtagcgtca gcatcgccgt 7081 ttgcctgccg tactcgggta gcccttccct gacgaccacc gacgaaccga cgatgcgtgc 7141 gttcttcgat cgttacttcg gtggcctgcc gcgtgacgcg cgtgacgaaa tgctgcgtca 7201 gtttctggcg aagccgagca acgacctgat taacgtgcgc tctagcacct ttcactataa 7261 gggtaatgtg ctgttgctgg gtgatgctgc gcatgcgact gcgccgttcc tgggtcaggg 7321 tatgaacatg gcgctggagg acgcccgcac gtttgtcgag ctgctggacc gccaccaggg 7381 cgaccaagac aaagcctttc cggagttcac ggagctgcgc aaagtccagg cagacgcaat 7441 gcaagacatg gctcgcgcca actatgacgt tttgagctgc tcgaacccga tctttttcat 7501 gcgtgcgcgt tacacgcgtt acatgcattc caagtttccg ggcctgtatc cgccggatat 7561 ggccgagaaa ctgtacttta cgagcgagcc gtacgatcgt ctgcaacaaa tccagcgtaa 7621 acagaatgtt tggtacaaga ttggtcgcgt gaattgaaga tcattaagga ggtaaaaaaa 7681 atggagaacc gtgagccacc actgttgcca gcccgttgga gcagcgccta tgtctcttat 7741 tggagcccga tgctgccgga tgaccagctg accagcggct attgctggtt cgactatgaa 7801 cgtgacatct gtcgtattga cggcctgttc aatccgtgga gcgagcgtga tactggttat 7861 cgcctgtgga tgtcggaggt tggtaatgcg gccagcggcc gtacctggaa acaaaaagtc 7921 gcctatggtc gtgagcgtac cgccctgggt gaacagctgt gtgagcgtcc gctggatgat 7981 gagactggcc cttttgccga attgttcctg ccacgcgatg tcctgcgccg tctgggtgcc 8041 cgtcacattg gccgtcgcgt ggttctgggt cgcgaagcgg acggttggcg ttaccagcgc 8101 ccaggtaaag gtccgagcac cctgtacctg gatgcggcga gcggcactcc actgcgcatg 8161 gtcaccggcg atgaagcgtc gcgtgcaagc ctgcgtgatt ttccgaatgt gagcgaggcg 8221 gagatcccgg acgcggtttt cgcggccaag cgctaatact agtagcggcc gctgcag //