ProtParam User-provided sequence: 10 20 30 40 50 60 KLWVTVYYGV PVWKEATTTL FCASDAKAYD TEVHNVWATH ACVPTDPNPQ EVVLVNVTEN 70 80 90 100 110 120 FNMWKNDMVE QMHEDIISLW DQSLKPCVKL TPLCVSLKCT DLKNDTNTNS SSGRMIMEKG 130 140 150 160 170 180 EIKNCSFNIS TSIRGKVQKE YAFFYKLDII PIDNDTTSYK LTSCNTSVIT QACPKVSFEP 190 200 210 220 230 240 IPIHYCAPAG FAILKCNNKT FNGTGPCTNV STVQCTHGIR PVVSTQLLLN GSLAEEEVVI 250 260 270 280 290 300 RSVNFTDNAK TIIVQLNTSV EINCTRPNNN TRKRIRIQRG PGRAFVTIGK IGNMRQAHCN 310 320 330 340 350 360 ISRAKWNNTL KQIASKLREQ FGNNKTIIFK QSSGGDPEIV THSFNCGGEF FYCNSTQLFN 370 380 390 400 410 420 STWFNSTWST EGSNNTEGSD TITLPCRIKQ IINMWQKVGK AMYAPPISGQ IRCSSNITGL 430 440 450 460 470 LLTRDGGNSN NESEIFRPGG GDMRDNWRSE LYKYKVVKIE PLGVAPTKAK RRVVQREKR Number of amino acids: 479 Molecular weight: 53692.2 Theoretical pI: 9.10 Amino acid composition:  Ala (A) 21 4.4% Arg (R) 23 4.8% Asn (N) 43 9.0% Asp (D) 17 3.5% Cys (C) 18 3.8% Gln (Q) 18 3.8% Glu (E) 24 5.0% Gly (G) 29 6.1% His (H) 7 1.5% Ile (I) 36 7.5% Leu (L) 26 5.4% Lys (K) 34 7.1% Met (M) 9 1.9% Phe (F) 18 3.8% Pro (P) 22 4.6% Ser (S) 36 7.5% Thr (T) 43 9.0% Trp (W) 10 2.1% Tyr (Y) 11 2.3% Val (V) 34 7.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0% Total number of negatively charged residues (Asp + Glu): 41 Total number of positively charged residues (Arg + Lys): 57 Atomic composition: Carbon C 2357 Hydrogen H 3737 Nitrogen N 667 Oxygen O 713 Sulfur S 27 Formula: C2357H3737N667O713S27 Total number of atoms: 7501 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 72515 Abs 0.1% (=1 g/l) 1.351, assuming all pairs of Cys residues form cystines Ext. coefficient 71390 Abs 0.1% (=1 g/l) 1.330, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is K (Lys). The estimated half-life is: 1.3 hours (mammalian reticulocytes, in vitro). 3 min (yeast, in vivo). 3 min (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 38.70 This classifies the protein as stable. Aliphatic index: 75.45 Grand average of hydropathicity (GRAVY): -0.398