LOCUS AY562544 9342 bp DNA circular SYN 05-APR-2004 DEFINITION Binary Vector pORE_R1, complete sequence. ACCESSION AY562544 VERSION AY562544.1 GI:45862381 KEYWORDS . SOURCE Binary vector pORE_R1 ORGANISM Binary vector pORE_R1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9342) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE pORE: a new series of binary vectors with a removable selectable marker and 21 site MCS JOURNAL Unpublished REFERENCE 2 (bases 1 to 9342) AUTHORS Coutu,C.M., Brandle,J., Brown,K., Simmonds,J., Brown,D., Mici,B. and Hegedus,D.D. TITLE Direct Submission JOURNAL Submitted (01-MAR-2004) Molecular Genetics, Agriculture and Agrifood Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada FEATURES Location/Qualifiers source 1..9342 /organism="Binary vector pORE_R1" /mol_type="other DNA" /db_xref="taxon:267421" /lab_host="Agrobacterium tumefaciens" terminator 1..253 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" misc_recomb 260..307 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" misc_feature 356..3610 /note="derived from pCB301 in GenBank Accession Number AF139061; used SDM to remove NotI, XhoI, EcoRV and SfiI sites" misc_feature 356..516 /note="T-DNA left border" rep_origin 523..1152 /note="RK2 oriV" CDS 1364..2158 /note="neomycin-kanamycin phosphotransferase type III" /codon_start=1 /transl_table=11 /product="NptIII" /protein_id="AAS78963.1" /db_xref="GI:45862382" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" CDS 2457..3605 /note="derived from plasmid RK2" /codon_start=1 /transl_table=11 /product="TrfA" /protein_id="AAS78964.1" /db_xref="GI:45862383" /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG SMVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKK RKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALF TTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPI TFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLD RGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKL ETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" rep_origin 3663..4543 /note="ColE1; high copy number origin of replication" misc_feature 4549..4711 /note="T-DNA right border" primer_bind 4724..4746 /note="M13/pUC forward" /PCR_conditions="60 degree C annealing temperature" misc_feature 4747..4868 /note="multiple cloning site; selected unique sites: SfoI-SacII-HindIII-NheI-MfeI-PspOMI-AclI-XhoI-XbaI-StuI- BamHI-SmaI-EcoRI-SalI-NotI-ClaI-PstI-SacI" CDS 4869..6680 /note="beta-glucuronidase; removed EcoRV, MfeI, and MluI sites; derived from pBI121 in GenBank Accession Number AF485783" /codon_start=1 /transl_table=11 /product="GUS" /protein_id="AAS78965.1" /db_xref="GI:45862384" /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ" primer_bind complement(6703..6725) /note="M13/pUC reverse" /PCR_conditions="60 degree C annealing temperature" terminator 6732..6984 /note="NOS terminator; polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; derived from pBI121 in GenBank Accession Number AF485783" misc_recomb 6991..7038 /note="FRT site; excision of selectable marker cassette from transformed plants using FLP recombinase" promoter 7055..8531 /note="At4g15440 promoter; hydroperoxide lyase promoter; constitutive; high level expression of transgenes in dicots; active in Arabidopsis, tobacco, cauliflower, soybean, alfalfa, peach, white spruce, wheat, and grape; in Arabidopsis, expression has been detected in all tissues but the petals, style, and pollen; removed ClaI, SfoI, XhoI and MfeI sites" CDS 8540..9334 /note="neomycin phosphotransferase II; removed SfoI, PstI, and NcoI sites; repaired reduction-in-function mutation; derived from pBI121 in GenBank Accession Number AF485783" /codon_start=1 /transl_table=11 /product="NptII" /protein_id="AAS78966.1" /db_xref="GI:45862385" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 61 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 121 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 181 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 241 atgttactag atccctaggg aagttcctat tccgaagttc ctattctctg aaaagtatag 301 gaacttcttt gcgtattggg cgctcttggc ctttttggcc accggtcgta cggttaaaac 361 caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt 421 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca 481 caaaatcacc actcgataca ggcagcccat cagtccacta gacgctcacc gggctggttg 541 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga 601 agccgtgtgc gagacaccgc agccgccggc gttgtggata cctcgcggaa aacttggccc 661 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt 721 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa 781 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga 841 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat 901 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg 961 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc 1021 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg 1081 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc 1141 tcccggcccg ctctcgcgtt ggcagcatca cccataattg tggtttcaaa atcggctccg 1201 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt 1261 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt 1321 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat 1381 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat 1441 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac 1501 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg 1561 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag 1621 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca 1681 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga 1741 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt 1801 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa 1861 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt 1921 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt 1981 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg 2041 gtcgatcagg gaggatattg gggaagaaca gtatgtcgag ctattttttg acttactggg 2101 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta 2161 cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca 2221 agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat 2281 tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga 2341 ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc 2401 aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga 2461 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg 2521 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt 2581 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg 2641 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg 2701 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga 2761 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caagccgcgt 2821 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc 2881 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca 2941 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca 3001 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt 3061 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt 3121 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg 3181 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc 3241 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt 3301 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca 3361 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact 3421 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg 3481 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt 3541 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac 3601 gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct ttactgagat 3661 cctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttggatctc ctgtggttgg 4561 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta 4621 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat 4681 agtttaaact gaaggcggga aacgacaatc tgctagtgga tctcccagtc acgacgttgt 4741 aaaacgggcg ccccgcggaa agcttgctag ccaattgggg cccaacgttc tcgagttttt 4801 ctagaaggcc ttggatccac ccgggagaat tcgtcgactt tgcggccgca tcgatactgc 4861 aggagctcat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc 4921 tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg 4981 cgttacaaga aagccgggct attgctgtgc caggcagttt taacgatcag ttcgccgatg 5041 cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag 5101 gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt 5161 gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg 5221 tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac 5281 tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc 5341 agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca 5401 ccacgccgaa cacctgggtg gacgatatta ccgtggtgac gcatgtcgcg caagactgta 5461 accacgcttc tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg 5521 atgcggatca acaggtggtt gcaactggac aaggcactag cgggactttg caagtggtga 5581 atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc acagccaaaa 5641 gccagacaga gtgtgatatt tacccgcttc gcgtcggcat ccggtcagtg gcagtgaagg 5701 gcgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt cgtcatgaag 5761 atgcggactt gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac cacgcattaa 5821 tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct gaagagatgc 5881 tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct gtcggcttta 5941 acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag 6001 aggcagtcaa cggggaaact cagcaagcgc acttacaggc gattaaagag ctgatagcgc 6061 gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg gatacccgtc 6121 cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacccgtaaa ctcgacccga 6181 cccgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg 6241 atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt 6301 tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc 6361 agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca 6421 ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg 6481 atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc 6541 aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga 6601 agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc 6661 agcagggagg caaacaatga ggtacctttt actagtgata tccctgtgtg aaattgttat 6721 ccgctacgcg tgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg 6781 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta 6841 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat 6901 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg 6961 cggtgtcatc tatgttacta gatcccatgg gaagttccta ttccgaagtt cctattctct 7021 gaaaagtata ggaacttcag cgatcgcaga cgtcaacgtg gatacttggc agtggttact 7081 tggcttttcc tttattttct tttggacgga agcggtggtt actttgtcac acatttaaaa 7141 aaacacgtgt ttctcacttt tttctattcc cgtcacaaac aattttaaga aagatccatc 7201 tatcgtgatc tttctatcaa acaaaagaaa aaaggtcttc atagtaacgc tacaacatca 7261 aatatgtggt tgctctgaca tcagtcggga aaataaggat atggcggcat tggccacatc 7321 tattggggtc ccaacttcct ttcacaaaaa aattaaattg ggtgtcccaa cttttatctt 7381 tgatatagtg acatgagtat cgggagcatt ggacaatgga taaaatgaga actaaaaaaa 7441 ttctggttaa tttttgatca ttgttattta aaaggttatt ttatctataa tctacccata 7501 ttgatcagtt ttatttaaat ttgtttagct accgctccac gagagagatc ctcatcttaa 7561 aaatggaata tggaaattac acacgacccc aaaagtatat tttttctctg gagaatgcta 7621 tttagagctt tgactatatg gtctgaatta gaaagacggg aaataaaatc tgctaagtga 7681 tataagctct aagtaggcga tgtgtgatgg agaacacctt ttctttaaca gtcttcatgt 7741 tttacagatt cgcgaacttc gaatatccct atacggtctg tctaaccctc gtgtgtcttt 7801 tgagtccaag ataaaggcca ttattgagta acatagacat gctggaatcc aaccattgaa 7861 gtcacaactg tccatgtaga ttctttggag aatctgaaaa gtcttaataa aggtggtgtt 7921 tcaaagaaaa caaaacaaat gagttaagaa aaaaaaatat catgtagtgg tcgagtatta 7981 tgttatttat tgtgtagcta ccaatcttta ttctttaaat ctgacataaa atgctacaaa 8041 ctttttacct cgtctatagc cccaaaaaac ctaaccacgg ttctaaaacc acacacagtg 8101 attttggttg acgacaatgc ctctccttcc tcaaaacgat ttatttacat tttttaaatc 8161 aaatgttaca ttttatacca taattaagtc tttttacaga atacttagat ggaagagatg 8221 tataaaaaag gaggaaattg taaaaaacat atttcgatca attaaaccag gattcataaa 8281 aatataagta tatatataaa tgatgtttcg tttagcgatg aacttcactc atatgataat 8341 acttaacaat ataagtacat aaaaaataaa ataaaattaa ttgtttacga aaagtctaca 8401 aatactgcat gtataattaa tgttctcttt atttatttat ttatacctta ccaagatata 8461 tctataaccg catagaaata gaaggcgaag agataatttc caaaaacaag aaaaacctct 8521 aagctcaaaa gggccggcca tgattgaaca agatggattg cacgcaggtt ctccggccgc 8581 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 8641 cgccgtgttc cggctgtcag cgcaggggag gccggttctt tttgtcaaga ccgacctgtc 8701 cggtgccctg aatgaacttc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 8761 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 8821 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 8881 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 8941 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 9001 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 9061 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgact catggcgatg cctgcttgcc 9121 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 9181 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 9241 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 9301 cgccttctat cgccttcttg acgagttctt ctgaggcgcg cc //