LOCUS       pT7_blank_Amp           4160 bp    DNA     circular SYN 06-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   siwong
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2024 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..63
                     /label=Custom promoter-RBS
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             34..56
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    50..63
                     /label=T7 (no RBS)
     misc_feature    63^64
                     /label=T7-RBS
     terminator      133..180
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      217..672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        699..803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(804..872,873..1664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1835..2423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2609..2751
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2853..3044)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 taatacgact cactataggc ctctagaaat aattttgttt aactttaaga aggagatata
       61 catttaatac ctaggctgct aaacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      121 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      181 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      361 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      421 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      541 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      661 cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      781 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      841 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      901 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      961 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1021 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1081 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1141 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1201 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1261 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1321 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     1381 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1441 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1501 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1561 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1621 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1681 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1741 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1801 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1861 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1921 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1981 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2041 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2101 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2161 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2221 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2281 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2341 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2401 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2461 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2521 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2581 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     2641 gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata
     2701 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     2761 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2821 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2881 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     2941 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     3001 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     3061 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     3121 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     3181 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     3241 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     3301 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     3361 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     3421 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     3481 aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc
     3541 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     3601 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     3661 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
     3721 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     3781 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     3841 attgcgttgc gccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     3901 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     3961 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     4021 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     4081 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     4141 agatctcgat cccgcgaaat
//
