LOCUS       pT7_blank_Kan           3320 bp    DNA     circular SYN 11-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   siwong
  TITLE     Direct Submission
  JOURNAL   Exported Dec 11, 2024 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..63
                     /label=Custom promoter-RBS
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             34..56
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    50..63
                     /label=T7 (no RBS)
     misc_feature    63^64
                     /label=T7-RBS
     terminator      133..180
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      217..672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(765..1580)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1702..2290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2476..2618
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2720..2911)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 taatacgact cactataggc ctctagaaat aattttgttt aactttaaga aggagatata
       61 catttaatac ctaggctgct aaacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      121 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      181 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      361 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      421 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      541 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      661 cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      721 ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg
      781 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
      841 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
      901 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
      961 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1021 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1081 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     1141 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     1201 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     1261 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     1321 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     1381 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     1441 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     1501 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     1561 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1621 attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1681 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1741 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1801 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1861 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1921 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1981 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2041 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2101 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2161 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2221 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2281 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2341 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2401 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2461 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2521 ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     2581 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     2641 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2701 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     2761 tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     2821 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     2881 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt
     2941 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg
     3001 atgaacatgc cgatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     3061 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     3121 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     3181 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     3241 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     3301 cgatctcgat cccgcgaaat
//
