LOCUS Exported 2161 bp ds-DNA circular SYN 15-NOV-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2161) AUTHORS Twistbioscience TITLE Direct Submission JOURNAL Exported Nov 15, 2017 from SnapGene 4.1.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2161 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..21 /label=Uni_9 R primer_bind complement(57..73) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(253..798) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." protein_bind complement(890..914) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS complement(1014..1874) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1875..1979) /gene="bla" /label=AmpR promoter primer_bind 2093..2109 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 2140..2161 /label=Uni_9 F ORIGIN 1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca 61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat 121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac 181 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc 241 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc 301 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg 361 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg 421 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc 481 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc 541 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt 601 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt 661 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga 721 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc 781 agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc 841 gccgtccatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt 901 ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa 961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1141 gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 1981 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc 2041 tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg 2101 acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc 2161 t //