LOCUS       Exported                2161 bp ds-DNA     circular SYN 15-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Twistbioscience
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2017 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..21
                     /label=Uni_9 R
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(253..798)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    complement(890..914)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1014..1874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1875..1979)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2093..2109
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2140..2161
                     /label=Uni_9 F
ORIGIN
        1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
      121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac
      181 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
      241 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
      301 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
      361 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
      421 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
      481 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
      601 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
      661 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
      721 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
      781 agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc
      841 gccgtccatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt
      901 ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa
      961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1141 gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1981 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc
     2041 tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg
     2101 acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc
     2161 t
//
