LOCUS       Exported                1953 bp ds-DNA     circular SYN 15-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Twistbioscience
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2017 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..21
                     /label=Uni_9 R
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(253..798)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      906..1000
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1021..1680)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1681..1783)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1885..1901
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1932..1953
                     /label=Uni_9 F
ORIGIN
        1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
      121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac
      181 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
      241 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
      301 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
      361 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
      421 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
      481 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
      601 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
      661 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
      721 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
      781 agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc
      841 gccgtccatg ggtagggggc ttcaaatcgt ccccccatac gatataagtt gttactagtg
      901 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      961 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1021 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1081 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1141 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1201 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1261 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1321 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1381 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1441 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1501 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1561 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1621 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1681 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1741 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attgatacct
     1801 gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc
     1861 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca
     1921 tctggaataa ggaagtgcca ttccgcctga cct
//
