LOCUS Exported 1953 bp ds-DNA circular SYN 15-NOV-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1953) AUTHORS Twistbioscience TITLE Direct Submission JOURNAL Exported Nov 15, 2017 from SnapGene 4.1.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1953 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..21 /label=Uni_9 R primer_bind complement(57..73) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(253..798) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." terminator 906..1000 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1021..1680) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" promoter complement(1681..1783) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind 1885..1901 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 1932..1953 /label=Uni_9 F ORIGIN 1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca 61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat 121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac 181 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc 241 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc 301 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg 361 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg 421 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc 481 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc 541 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt 601 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt 661 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga 721 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc 781 agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc 841 gccgtccatg ggtagggggc ttcaaatcgt ccccccatac gatataagtt gttactagtg 901 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga 961 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa 1021 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac 1081 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc 1141 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc 1201 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt 1261 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga 1321 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt 1381 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc 1441 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg 1501 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat 1561 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg 1621 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat 1681 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct 1741 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attgatacct 1801 gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc 1861 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca 1921 tctggaataa ggaagtgcca ttccgcctga cct //