LOCUS Exported 2105 bp ds-DNA circular SYN 15-NOV-2017 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2105) AUTHORS Twistbioscience TITLE Direct Submission JOURNAL Exported Nov 15, 2017 from SnapGene 4.1.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2105 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..21 /label=Uni_9 R primer_bind complement(57..73) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(253..798) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS complement(909..1724) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1845..1861 /label=SK primer /note="common sequencing primer, one of multiple similar variants" terminator 1862..1908 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 2037..2053 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 2084..2105 /label=Uni_9 F ORIGIN 1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca 61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat 121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac 181 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc 241 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc 301 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg 361 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg 421 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc 481 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc 541 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt 601 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt 661 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga 721 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc 781 agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc 841 gccgtccatg ggtagggggc ttcaaatcgt ccgctctgcc agtgttacaa ccaattaaca 901 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga 961 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg 1021 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca 1081 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga 1141 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca 1201 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt 1261 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca 1321 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa 1381 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac 1441 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc 1501 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt 1561 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat 1621 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt 1681 aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta 1741 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg 1801 taacatcaga gattttgaga cacaacgtgg ctttcccccg ccgctctaga actagtggat 1861 ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1921 gcattatacg agacgtccag gttgggatac ctgaaacaaa acccatcgta cggccaagga 1981 agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac gacgttgtaa 2041 aacgacggcc agtcatgcat aatccgcacg catctggaat aaggaagtgc cattccgcct 2101 gacct //