LOCUS       Exported                2105 bp ds-DNA     circular SYN 15-NOV-2017
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Twistbioscience
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2017 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..21
                     /label=Uni_9 R
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(253..798)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(909..1724)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1845..1861
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1862..1908
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2037..2053
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2084..2105
                     /label=Uni_9 F
ORIGIN
        1 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
      121 ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac
      181 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
      241 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
      301 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
      361 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
      421 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
      481 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
      601 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
      661 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
      721 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
      781 agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc
      841 gccgtccatg ggtagggggc ttcaaatcgt ccgctctgcc agtgttacaa ccaattaaca
      901 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
      961 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     1021 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     1081 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     1141 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     1201 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     1261 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     1321 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     1381 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     1441 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     1501 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     1561 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     1621 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     1681 aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     1741 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     1801 taacatcaga gattttgaga cacaacgtgg ctttcccccg ccgctctaga actagtggat
     1861 ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1921 gcattatacg agacgtccag gttgggatac ctgaaacaaa acccatcgta cggccaagga
     1981 agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac gacgttgtaa
     2041 aacgacggcc agtcatgcat aatccgcacg catctggaat aaggaagtgc cattccgcct
     2101 gacct
//
