LOCUS       pTwist_CMV_mIgL1        6951 bp    DNA     circular SYN 04-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(1175..6951,1..1174)
                     /organism="pTwistCMVmIgL1 in pTwist CMV BG WPRE Neo v2.0"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          642..1117
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    1174^1175
                     /label=Your gene here
     misc_feature    1175..1492
                     /label=mIgL1
     misc_feature    1517..2105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(1988..1999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2158..2279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2394..2440
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    2550..2621
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        2666..2862
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2713..2848
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2876..3670
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3681..3905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4101..4689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5435..5539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5540..5608,5609..6400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6413..6549
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6694..6765
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     polyA_signal    6823..6871
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tcccctcgaa
      601 gcttacatgt ggtaccgagc tcggatcctg agaacttcag ggtgagtcta tgggaccctt
      661 gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta
      721 acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct
      781 ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc
      841 tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag
      901 tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt ctgcatataa
      961 attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc
     1021 tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt
     1081 gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg tgctggtctg
     1141 tgtgctggcc catcactttg gcaaaggcgg ccgccagcct aagagcagcc ccagcgtgac
     1201 actgtttcct ccaagcagcg aggaactcga gacaaacaag gccacactcg tgtgcaccat
     1261 caccgacttc tatcccggcg tggtcaccgt ggattggaag gtggacggaa cccctgtgac
     1321 acaaggcatg gaaaccacac agcccagcaa gcagagcaac aacaagtaca tggccagcag
     1381 ctacctgaca ctgaccgcca gagcctggga gagacacagc tcctacagct gccaagtgac
     1441 acacgagggc cacaccgtgg aaaagtctct gagcagagcc gactgcagct gaatcgattt
     1501 ctggatccgc aggcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1561 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     1621 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     1681 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     1741 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     1801 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     1861 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     1921 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     1981 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2041 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     2101 cccgcctgtc tagcttgact gactgagata cagcgtacct tcagctcaca gacatgataa
     2161 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2221 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2281 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     2341 aaagcaagta aaacctctac aaatgtggta ttggcccatc tctatcggta tcgtagcata
     2401 accccttggg gcctctaaac gggtcttgag gggttttttg tgcccctcgg gccggattgc
     2461 tatctaccga ggcactggtg tactgataga gccgggctag atgttaactg tcagctatcc
     2521 tgcaggcgat ctctcgattt cgatcaagac attcctttaa tggtcttttc tggacaccac
     2581 taggggtcag aagtagttca tcaaactttc ttccctccct aatctcattg gttaccttgg
     2641 gctatcgaaa cttaattaag cgatctgcat ctcaattagt cagcaaccat agtcccgccc
     2701 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatcgc
     2761 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2821 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaggaggtag ccaacatgat
     2881 tgaacaagat ggattgcacg caggttctcc cgccgcttgg gtggagaggc tattcggcta
     2941 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3001 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccagga
     3061 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3121 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3181 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3241 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3301 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3361 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga
     3421 ggatctcgtc gtgacccacg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     3481 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     3541 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     3601 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     3661 gttcttctag tatgtaagcc ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3721 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3781 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3841 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     3901 tatggttaat taaccagtca agtcagctac ttggcgagat cgacttgtct gggtttcgac
     3961 tacgctcaga attgcgtcag tcaagttcga tctggtcctt gctattgcac ccgttctccg
     4021 attacgagtt tcatttaaat catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4261 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4501 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4561 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4681 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4741 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4801 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4861 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4921 gttgcattta aatttccgaa ctctccaagg ccctcgtcgg aaaatcttca aacctttcgt
     4981 ccgatccatc ttgcaggcta cctctcgaac gaactatcgc aagtctcttg gccggccttg
     5041 cgccttggct attgcttggc agcgcctatc gccaggtatt actccaatcc cgaatatccg
     5101 agatcgggat cacccgagag aagttcaacc tacatcctca atcccgatct atccgagatc
     5161 cgaggaatat cgaaatcggg gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt
     5221 ctcgacgatc catatcgttg cttggcagtc agccagtcgg aatccagctt gggacccagg
     5281 aagtccaatc gtcagatatt gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa
     5341 acctagatat tgatagtctg atcggtcaac gtataatcga gtcctagctt ttgcaaacat
     5401 ctatcaagag acaggatcag caggaggctt tcgccgcgga acccctattt gtttattttt
     5461 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5521 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5581 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5641 tgaagatcag ttgggtgcgc gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5701 ccttgagagt tttcgccccg aagaacgctt tccaatgatg agcactttta aagttctgct
     5761 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5821 ctattctcag aatgacttgg ttgagtattc accagtcaca gaaaagcatc ttacggatgg
     5881 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5941 cttacttctg acaacgattg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6001 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6061 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacc ttgcgtaaac tattaactgg
     6121 cgaactactt actctagctt cccggcaaca gttgatagac tggatggagg cggataaagt
     6181 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6241 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6301 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6361 gatcgctgag ataggtgcct cactgattaa gcattggtaa ccgattctag gtgcattggc
     6421 gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca
     6481 gcaacggata cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt
     6541 tatccttaag atcgtttaaa ctcgactctg gctctatcga atctccgtcg tttcgagctt
     6601 acgcgaacag ccgtggcgct catttgctcg tcgggcatcg aatctcgtca gctatcgtca
     6661 gcttaccttt ttggcagcga tcgcggctcc cgacatcttg gaccattagc tccacaggta
     6721 tcttcttccc tctagtggtc ataacagcag cttcagctac ctctcaattt gcgtatagag
     6781 cgggtcatcg aagggttata cactcgtaat cgttgcgtta cacacacaaa aaaccaacac
     6841 acatccatct tcgatggata gcgattttat tatctaactg ctgatcgagt gtagccagat
     6901 ctagtaatca attacggggt cattagttca tagcccatat atggagttcc g
//
