LOCUS       Exported                2421 bp ds-DNA     circular SYN 26-MAR-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Twistbioscience
  TITLE     Direct Submission
  JOURNAL   Exported Mar 27, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
COMMENT     Insert is cloned directly after 3' C in the minimal Kozac site as 
            follows CACC INSERT GAC
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    253..256
                     /label=Minimal Kozac
     gap             257..306
                     /estimated_length=50
     gene            257..306
                     /label=Your Gene Inserted Here
     protein_bind    complement(308..407)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(464..480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(652..1240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1376..2191)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      2329..2375
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc tgcaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnngacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      361 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgagg ctaggtggag
      421 gctcagtgat gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg
      481 tgtgaaattg ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca
      541 agacattagg tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc
      601 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      661 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      721 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      781 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      841 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      901 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      961 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1021 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1081 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1141 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1201 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1261 tacggggtct gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt
     1321 agggggcttc aaatcgtccg ctctgccagt gttacaacca attaacaaat tctgattaga
     1381 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     1441 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     1501 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     1561 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     1621 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     1681 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     1741 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     1801 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     1861 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     1921 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     1981 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     2041 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     2101 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     2161 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     2221 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     2281 tttgagacac aacgtggctt tcccccgccg ctctagaact agtggatcca aataaaacga
     2341 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca ttatacgaga
     2401 cgtccaggtt gggatacctg a
//
