LOCUS       Exported                2415 bp ds-DNA     circular SYN 22-MAR-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Twistbioscience
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
COMMENT     Insert is cloned directly after 3' C in the minimal Kozac site CACC 
            Insert GAC
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     gap             252..301
                     /estimated_length=50
     gene            252..301
                     /label=Your Gene Inserted Here
     protein_bind    complement(302..401)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(458..474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(646..1234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1370..2185)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      2323..2369
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
      361 aacgaacagg tcactatcag tcaaaataaa atcattattt gaggctaggt ggaggctcag
      421 tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa
      481 attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat
      541 taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg
      601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      841 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1081 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1261 gtctgacgct ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg
     1321 cttcaaatcg tccgctctgc cagtgttaca accaattaac aaattctgat tagaaaaact
     1381 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     1441 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     1501 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     1561 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     1621 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     1681 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     1741 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     1801 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     1861 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     1921 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     1981 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     2041 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     2101 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag
     2161 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     2221 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     2281 acacaacgtg gctttccccc gccgctctag aactagtgga tccaaataaa acgaaaggct
     2341 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cgcattatac gagacgtcca
     2401 ggttgggata cctga
//
