LOCUS       pTwist_IVT_hBG_5        2549 bp    DNA     circular SYN 06-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   siwong
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2024 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..75
                     /label=uni9-F
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    95..97
                     /label=Transcription start sequence
     5'UTR           98..147
                     /label=hBG 5' UTR
     misc_feature    147^148
                     /label=Your gene here
     3'UTR           148..281
                     /label=hBG 3' UTR
     misc_feature    282..331
                     /label=PolyA tail
     misc_feature    333..363
                     /label=Multiple linearization enzyme sites
     primer_bind     complement(364..384)
                     /label=Uni9-R
     primer_bind     complement(420..436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(608..1196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1377..2186)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataagtct agagaagtgc
       61 cattccgcct gaccttaata cgactcacta taggaggaca tttgcttctg acacaactgt
      121 gttcactagc aacctcaaac agacaccgct cgctttcttg ctgtccaatt tctattaaag
      181 gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg gccttgagca
      241 tctggattct gcctaataaa aaacatttat tttcattgca aaaaaaaaaa aaaaaaaaaa
      301 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa acgaagagct ctagagcgct gtttaaacac
      361 gtgaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg
      421 tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc
      481 tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg
      541 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1021 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1141 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1321 gaagttttaa atcaagccca atctgaataa tgttacaacc aattaaccaa ttctgattag
     1381 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     1441 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     1501 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     1561 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     1621 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     1681 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     1741 tgagcaagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     1801 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     1861 tctaatacct ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca tgcatcatca
     1921 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     1981 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     2041 tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg cccgacatta
     2101 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     2161 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     2221 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     2281 gacacgggcc agagctgcat cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     2341 cagctcccgg gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     2401 agggcgcgtc agcgggtgtt ggctgttgtt tgtcgcatta tacgagcgtc caggttggga
     2461 tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg atccaccaca
     2521 agcgccaggg ttttcccagt cacgacgtt
//
