LOCUS       pTwist_Kan_HC_v2        2255 bp DNA     circular SYN 25-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   siwong
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(87..675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(856..1665)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2029..2045
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2076..2097
                     /label=Uni_9 F
     misc_feature    2097^2098
                     /label=Your gene here
     primer_bind     complement(2098..2118)
                     /label=Uni_9 R
     primer_bind     complement(2154..2170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
       61 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      661 aaaaaaggat ctcaagaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
      721 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
      781 atccttttaa attaaaaatg aagttttaaa tcaagcccaa tctgaataat gttacaacca
      841 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
      901 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
      961 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     1021 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1081 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     1141 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     1201 attcattcgt gattgcgcct gagcaagacg aaatacgcga tcgctgttaa aaggacaatt
     1261 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     1321 acctgaatca ggatattctt ctaatacctg gaatgctgtt tttccgggga tcgcagtggt
     1381 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     1441 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     1501 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc
     1561 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     1621 ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     1681 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     1741 gtaacatcag agattttgag acacgggcca gagctgcatc gcgcgtttcg gtgatgacgg
     1801 tgaaaacctc tgacacatgc agctcccggg acggtcacag cttgtctgta agcggatgcc
     1861 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gctgttgttt gtcgcattat
     1921 acgagcgtcc aggttgggat acctgaaaca aaacccatcg tacggccaag gaagtctcca
     1981 ataactgtga tccaccacaa gcgccagggt tttcccagtc acgacgttgt aaaacgacgg
     2041 ccagtcatgc ataatccgca cgcatctgga ataaggaagt gccattccgc ctgacctagg
     2101 ctaggtggag gctcagtgat gataagtctg cgatggtgga tgcatgtgtc atggtcatag
     2161 ctgtttcctg tgtgaaattg ttatccgctc agagggcaca atcctattcc gcgctatccg
     2221 acaatctcca agacattagg tggagttcag ttcgg
//
