LOCUS       Exported                7520 bp ds-DNA     circular SYN 30-NOV-2018
DEFINITION  Cloning Sites 5'-3'
            BamHI MluI
            BamHI SpeI
            BamHI XhoI
            MluI SpeI
            MluI XhoI
            SpeI XhoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2018 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="E.coli"
                     /strain="XL1 Blue"
                     /mol_type="plasmid DNA"
     promoter        9..580
                     /label=CMV
                     /note="CMV Promoter"
     misc_feature    582..676
                     /label=HIV R
     misc_feature    677..761
                     /label=HIV U5
     CDS             809..944
                     /label=psi
     CDS             864..875
                     /label=Major splice donor
     misc_feature    913..1251
                     /label=Delta GAG
     misc_feature    1275..2132
                     /label=Rev Response Element
     promoter        2326..2768
                     /label=SFFV
                     /note="SFFV"
     misc_feature    2840..2871
                     /label=Lentivirus Cloning Site
     misc_feature    2942..3492
                     /label=EMCV IRES
     CDS             3495..4094
                     /label=Puromycin
     misc_feature    4102..4112
                     /label=Stop codons x 3
     misc_feature    4121..4697
                     /label=WPRE
     misc_feature    4794..4848
                     /label=U3
     misc_feature    4850..4944
                     /label=HIV R
     misc_feature    4945..5029
                     /label=HIV U5
     polyA_site      5031..5261
                     /label=Rb Alpha-1-Globin pA
     rep_origin      5276..5457
                     /label=SV40 Origin
     rep_origin      5482..6366
                     /label=pMB1 (Bacterial, 500-800 copies)
     CDS             5542..6130
                     /label=pMB1 Origin
     CDS             6492..7352
                     /label=Ampicillin (AmpR)
     terminator      7366..7502
                     /note="RrnG Bacterial Terminator"
ORIGIN
        1 gcgatcgcag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctcgttt agtgaaccgg ggtctctctg gttagaccag
      601 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      661 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      721 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggacc
      781 tgaaagcgaa agggaaacca gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      841 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      901 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      961 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1021 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1081 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1141 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1201 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1261 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1321 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1381 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1441 ttcttgggag cagcaggaag cactatgggc gcagcctcaa tgacgctgac ggtacaggcc
     1501 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1561 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1621 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1681 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1741 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1801 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1861 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1921 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1981 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2041 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2101 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2161 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2221 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttgg ctcccgatcg
     2281 ttgcgttaca cacacaatta ctgctgatcg agtgtagcct tcgaatgaaa gaccccacct
     2341 gtaggtttgg caagatagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     2401 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     2461 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     2521 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     2581 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     2641 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     2701 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgattgactg
     2761 agtcgccctg atcattgtcg atcctaccat ccactcgaca cacccgccag ggcccgcatc
     2821 caccatcgca gacttatcat ggatcctgac gcgtgtacta gtgtctcgag cttattccag
     2881 atgcgtgcgg atggaattcg agctcggtac catgccaaaa gcaaagcgct atcgcgcctt
     2941 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     3001 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     3061 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     3121 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     3181 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     3241 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     3301 aaagagtcaa atggctcccc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     3361 taccccattg tatgggatct gatctggggc ctcggtgcac atgcttttca tgtgtttagt
     3421 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     3481 acgatgataa taacatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     3541 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     3601 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     3661 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     3721 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     3781 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggagggcctc ctggcgccgc
     3841 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     3901 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggccgccgag cgcgccgggg
     3961 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     4021 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     4081 agcccggtgc ctaggctagc ttgactgact gagtcgacaa tcaacctttt ggattacaaa
     4141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4501 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4681 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa
     4741 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4801 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt cctggccaac
     5041 gtgagcaccg tgctgacctc caaatatcgt taagctggag cctgggagcc ggcctggccc
     5101 tccgcccccc ccacccccgc agcccacccc tggtctttga ataaagtctg agtgagtggc
     5161 cgacagtgcc cgtggagttc tcgtgacctg aggtgcaggg ccggcgctag ggacacgtcc
     5221 gtgcacgtgc cgaggccccc tgtgcagctg caagggacag gcctagccct gcaggcctaa
     5281 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgcct catggctgac
     5341 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5401 agtgaggacg cttttttgga ggccgaggct tttgcaaaga tcgaacaaga gacaggacct
     5461 gcaggttaat taaatttaaa tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5941 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6361 agttgcattt aaatggccgg cctggcgcgc cgtttaaacc tagatattga tagtctgatc
     6421 ggtcaacgta taatcgagtc ctagcttttg caaacatcta tcaagagaca ggatcagcag
     6481 gaggctttcg catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6541 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6601 agttgggtgc gcgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6661 gttttcgccc cgaagaacgc tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6721 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6781 agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6841 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6901 tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6961 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7021 acaccacgat gcctgtagca atggcaacaa ccttgcgtaa actattaact ggcgaactac
     7081 ttactctagc ttcccggcaa cagttgatag actggatgga ggcggataaa gttgcaggac
     7141 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7201 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7261 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7321 agataggtgc ctcactgatt aagcattggt aaccgattct aggtgcattg gcgcagaaaa
     7381 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
     7441 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
     7501 agatcccgaa tcgtttaaac
//
