LOCUS       Exported                5701 bp ds-DNA     circular SYN 30-NOV-2018
DEFINITION  Cloning Sites 5'-3'
            BamHI MluI
            BamHI SpeI
            BamHI XhoI
            MluI SpeI
            MluI XhoI
            SpeI XhoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Genetic Engineering
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2018 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        9..580
                     /note="CMV Promoter"
     misc_feature    582..676
                     /label=HIV R
     misc_feature    677..761
                     /label=HIV U5
     misc_feature    762..779
                     /label=Primer Binding Site
     CDS             809..944
                     /label=psi
     CDS             864..875
                     /label=Major splice donor
     misc_feature    913..1251
                     /label=Delta GAG
     misc_feature    1275..2132
                     /label=Rev Response Element
     promoter        2326..2768
                     /label=SFFV promoter
                     /note="SFFV"
     misc_feature    2810..2839
                     /label=Lentivirus Cloning Site
     misc_feature    2975..3029
                     /label=U3
     misc_feature    3031..3125
                     /label=HIV R
     misc_feature    3126..3210
                     /label=HIV U5
     polyA_site      3212..3442
                     /label=Rb Alpha-1-Globin pA
     rep_origin      3457..3638
                     /label=SV40 Origin
     rep_origin      3663..4547
                     /label=pMB1 (Bacterial, 500-800 copies)
     CDS             3723..4311
                     /label=pMB1 Origin
     CDS             4673..5533
                     /label=Ampicillin (AmpR)
     terminator      5547..5683
                     /note="RrnG Bacterial Terminator"
ORIGIN
        1 gcgatcgcag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctcgttt agtgaaccgg ggtctctctg gttagaccag
      601 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      661 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      721 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggacc
      781 tgaaagcgaa agggaaacca gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      841 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      901 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      961 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1021 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1081 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1141 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1201 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1261 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1321 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1381 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1441 ttcttgggag cagcaggaag cactatgggc gcagcctcaa tgacgctgac ggtacaggcc
     1501 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1561 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1621 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1681 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1741 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1801 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1861 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1921 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1981 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2041 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2101 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2161 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2221 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttgg ctcccgatcg
     2281 ttgcgttaca cacacaatta ctgctgatcg agtgtagcct tcgaatgaaa gaccccacct
     2341 gtaggtttgg caagatagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     2401 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     2461 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     2521 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     2581 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     2641 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     2701 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgattgactg
     2761 agtcgccctg atcattgtcg atcctaccat ccactcgaca cacccgccag gatcctgacg
     2821 cgtgtactag tgtctcgagg gcccgcatcc accatcgcag acttatcatc ttattccaga
     2881 tgcgtgcgga tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     2941 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3001 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     3061 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     3121 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3181 cttttagtca gtgtggaaaa tctctagcag tcctggccaa cgtgagcacc gtgctgacct
     3241 ccaaatatcg ttaagctgga gcctgggagc cggcctggcc ctccgccccc cccacccccg
     3301 cagcccaccc ctggtctttg aataaagtct gagtgagtgg ccgacagtgc ccgtggagtt
     3361 ctcgtgacct gaggtgcagg gccggcgcta gggacacgtc cgtgcacgtg ccgaggcccc
     3421 ctgtgcagct gcaagggaca ggcctagccc tgcaggccta actccgccca tcccgcccct
     3481 aactccgccc agttccgccc attctccgcc tcatggctga ctaatttttt ttatttatgc
     3541 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggac gcttttttgg
     3601 aggccgaggc ttttgcaaag atcgaacaag agacaggacc tgcaggttaa ttaaatttaa
     3661 atcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4141 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4261 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4321 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4381 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4441 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4501 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcatt taaatggccg
     4561 gcctggcgcg ccgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     4621 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat
     4681 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4741 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg
     4801 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4861 ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4921 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4981 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5041 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     5101 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5161 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5221 aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag cttcccggca
     5281 acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5341 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5401 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5461 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5521 taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     5581 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     5641 ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa
     5701 c
//
