LOCUS       pUC19        2686 bp DNA     circular SYN 22-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   siwong
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             111..434
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    128..157
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(185..201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        675..779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(780..848,849..1640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1811..2399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
      181 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      241 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      301 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
      361 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
      421 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      481 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
      541 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
      601 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      661 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      721 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      781 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      841 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      901 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      961 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1021 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1081 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1141 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1201 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1261 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1321 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1381 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1441 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1501 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1561 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1621 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1681 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1741 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1921 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2461 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2521 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2581 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2641 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaat
//
